Kusvika pari zvino, PCR kuongororwa inoonekwa seimwe yenzira dzakavimbika dzekuongorora zvirwere zvakasiyana-siyana zvinotapukira. Mukuwedzera, iyo nzira inowedzera kuwanika. Pamusana pehupamhi hwehuwandu hwezvakananga, mukana wekutora mhinduro dzisina kururama hauna kubviswa.
Method of analysis
Panguva yekuongororwa, nyaya yekuongorora inowanikwa mune chiridzwa chakakosha. Wedzera enzyme iyo inobatanidzwa mukugadzirwa kwemajini. Zvadaro pane kuinyorwa kwakawanda kweDNA kana RNA yea causative agent wechirwere. Kubva pakurongedza kusvika kumhepo, nhamba yemakopi eDNA inowedzera kusvika pane iyo yakajeka kuziva iyo pathogen.
Kuongororwa kweropa uchishandisa nzira yePCR inowanzoshandiswa mumakiriniki ekudzidzira kuona chikonzero chinotapukira chechirwere. Izvo zvinokwanisikawo kudzidza urini, smear kubva pamutsipa nezvimwe zvinhu zvezvisikwa. Madzimai, nokuda kwekuongorora kwePCR, zvipfeko kubva munhengo dzepabonde, smear kubva kurerera , chirwere chechibereki chinoshandiswa. Zvakakosha kuziva kuti kugadzirira sei kuongororwa kwePCR kuvakadzi, kuitira kuti mugumisiro uve wakavimbika sezvinobvira. Chinhu chinokosha kuchengeta mitemo inotevera:
- kurega kuita zvepabonde mumazuva matatu vasati vatariswa;
- mazhinji mabhakitiriya anokwanisa "kushambidza" nemukaka, saka usazora mushure mokuedzwa;
- Usaita chiitiko pakarepo mushure mekuenda kumwedzi, unofanira kumirira mazuva matatu mushure mekupedzisira.
Pamberi pekuongorora ropa, hapana chigadziriro chaicho.
PCR - kuongorora kunoratidzei?
Zvinonyatsozivikanwa kuti PCR tsananguro inoratidza kuvapo kwezvirwere zvakasiyana-siyana zvehutachiona uye bhakitiriya. Iyi nzira inobatsirawo pakuonekwa kwemazuva mashoma, asingagoneki zvirwere. Kuongororwa kwepabonde uchishandisa nzira yePCR inoita kuti zvive nyore kuvhara pathogenic agent kunyange pakuvapo kwemasero asina ma virus uye mabhakitiriya. Zvakakosha kuziva kuti zvipi zvePCR zvinowanikwa mubako rezvirwere zvepabonde, izvi ndezvi:
- Chlamydia;
- mycoplasma ;
- ureaplasmas (parvum uye urealiticum);
- iyo causative agent we gonorrhea;
- marudzi akasiyana evanhu papillomavirus;
- Trichomonas;
- gardnerelles;
- Vadzidzi.
Nezvirwere zvinopararira zvemuviri wechipfu, izvo zvinyorwa zvePCR ndeye smear kubva munharaunda yemukango, urethra nemukadzi. Kugadzirira kuberekwa kunofanira kutaurirwa nemutoro mukuru. Paunenge uchironga pamuviri, PCR inoongorora inofanirwa mune zviitiko apo kune kusafungirwa kwezvirwere zvinotapurika zvakanyanya. Uye kana pane utachiona, zviri nani kutsiva pamuviri. Zvakakosha kuziva kuti kuedza kwekuziva zvirwere zvinoratidzwa pamusoro apa hazvifaniri kupedzerwa kwete kumukadzi chete, asiwo kumurume.
Uyewo, nzira yePCR inoratidza zvirwere zvinotevera:
- hepatitis B naC virasi;
- Mycobacterium tuberculosis;
- HIV;
- mavirisi emhuri yake herpes, kusanganisira Epstein-Barr utachiona uye cytomegalovirus;
- Helicobacter pylori chirwere.
Kutsanangurwa kwemigumisiro
Kugadziriswa kwekodzero yePCR hakuiti kukonzera. Kazhinji zvikamu zvekuongorora kwePCR zvinogona kuwanikwa seizvi:
- Mhedzisiro isina kunaka inoreva kuti uyo anotsvaga-anoshanda anoparadzanisa haana kuwanikwa mune zvinyorwa pasi pekudzidza.
- Chiitiko chakanaka chinoratidza kuvapo kweDNA kana RNA pathogen. Izvi zvinoreva kuti, nekunyatsoziva, zvinogona kupikiswa kuti ndiyo inoratidzika yehutachiona iyo ndiyo inokonzera chirwere ichi.
Mune zvimwe zviitiko, kuedza kwakakwana kwehutachiona hunoitwa. Izvi zvinonyanya kuitika kune zvirwere zvinokonzerwa nemashiripiti mashoma. Sezvo mabhakitiriya aya anoratidza maitiro avo akaipa apo iyo mari yakawanda. Uyewo, kuwanda kwePCR kuongorora kwakakosha pakusarudzwa kwemaitiro ekurapa uye nechinangwa chokurapa kurapwa kwezvirwere zvakadai seHIV uye hepatitis viruses.