Kuchengetedza chirume

Iyi nzira yekuchengetedza chirume (ejaculate), kufanana nekugadzirisa, yakanyanya kupararira mu cryomedicine. Inosanganisira kuwedzerwa kwemasvikiro chaiwo kusvika kumvura inonzi seminal fluid, uye kuwedzera kuisvibisa nekushandiswa kwemhepo inonzi nitrogen vaporo. Chimbofunga iyi nzira yekuchengetedza varume vemajeri masero muzvinyorwa zvakanyanya uye kukuudzai kuti mbeu yei inokodzera kubudisa nekuchengetedza zvakare.

Ko goropreservation inowedzera sei mikana yekugadzirwa?

Nekudyara kwepanyama, zvakavanzika zvezvinyorwa zvepabonde zvepabonde zvinosanganiswa pamwe chete nemasero epidermis, semugumisiro, imwe shanduko mu pH yepakati inosvika, iyo inotungamirira pakuparadzwa kwepopoprotein chivande che spermatozoa, i.e. kuti vashandise . Kuva mumamiriro ezvinhu aya, mararamiro emakamuri ekubereka anowedzera zvishoma, izvo zvisingabatsiri kushandiswa kwavo kweVFF. Ndicho chikonzero nzira yekunyororeswa inoshandiswa .

Iyi nzira inonyanya kuwedzera mararamiro emuviri wemunhu, uye inobvumira:

• kuderedza motokari yepermatozoa;
• unovapa neimwezve simba remasimba;
• inodzivirira utachiona hwemagetsi kubva pakuparadzwa;
• kunodzivirira kuwedzera kwezvinhu zvinokonzera tsvina;
• inodzivisa kubudiswa kwemakemikari mune ejaculate.

Ndeapi masvikiro anoshandiswa kuchengeta mbeu?

Kuti zvive nyore, izvo zvinodanwa kushandiswa kwekupwanya ejaculate, zvakakosha kuti kusarudzwa kwemajeri masero kubva mairi, tanga kuwedzera muviri wemhombwe. Zviratidzo zvakakosha zvinoshandiswa.

Kusvika iye zvino, zvinowanzoshandiswa pakugadzirwa kwemashizha kuitira kuchengetedza mbeu, chimwe nechimwe chinoda mamiriro akasiyana. Kazhinji, rudzi urwu rwezvakatipoteredza runosanganisira zvikamu zvakawanda, kazhinji zvinenge zvitatu. Saka, mumagariro avo zvinogona kugovera shuga, kazhinji uye inoshandiswa glucose uye lactose, sodium citrate.

Kana tichitaura nezvemamwe makemikari akawanda anogona kushandiswa sevhidhiyo yekuchengetedza chirume, ipapo pakati pazvo zvinogona kunzi Tris-buffer, Trilon B, EDTA, Spermosan PPK.

Ndeapi pasi pemamiriro ezvinhu ejaculate yakachengetwa?

Inyanzvi yekugadzira uye kuchengetedza mbeu inoda kuomerera kune humwe hutano hwekunakisa, pamwe nekushandiswa kwezvinhu zvakakosha. Muchiitiko ichi, imba ye laboratori pachayo inofanira kunge yakagadzirirwa nemichina yakasvibisa mhepo.

Pamberi pe ejaculate inounganidzwa, zvigadziro zvose zvinodiwa zvegyopreservation, kunyanya mhando dzose dzemafuta, zvidzidzo zvidzidzo, pipettes, mafiritsi emapepa anoviriswa mune imwe kambani yepamusoro, pane kutonhora kwe 130-150 degrees. Pamberi pezvinyorwa zvitsanangudzo zvakananga, dzinoiswa mune imwe thermostat chaiyo, iyo inochengetedza kugara kwechando chema 37 degrees.

Kamwe chete mukono wechirume uchibviswa, unoiswa mubhokisi risina kuchena. Ichando chekuchengetedza chemuti inofanira kugara chiripo. Inotonhorera inoitwa mumatanho maviri.

Pamusoro pekutanga kwavo, ejaculate inotanga kuiswa muimba inotonhora, umo kutonhora kunowira zvishoma nezvishoma. Sezvo mutemo, kukosha kwayo ndiko -35 degrees. Mushure meizvi, kubhururuka kwakadzika kunoitwa, kubhabhatidza bhorosi chaiye nehuta mumvura inonzi nitrogen. Muchiitiko ichi, hupenyu hwemafu ehuta hunokwanisa kusvika makumi emakumi emakore.

Kuti ushandise ejaculate, cryopreserved yapfuura, mudziyo pamwe chete nawo unowanikwa mumvura inodziya, uko kunononoka kunowanzoitika. Mushure mokunge, iyo yakashandiswa inonzi cryoprotectants inodzingwa, nekudzokororazve kuderedzwa kwehuta pa centrifuge. Pashure pacho, seminal fluid pachayo inotsiviwa nechirongwa chemuviri mukati umo spermatozoa inowanzoiswa.